Selected publications that include data collected on the Biophysics instrumentation:

Self-Assembly of Conjugated Metallopolymers with Tunable Length and Controlled Regiochemistry. Greenfield JL, Rizzuto FJ, Goldberga I, Nitschke JR. (2017) Angew Chem Int Ed Engl 56(26):7541-7545. PMID: 28470946.

Dissecting how modular polyketide synthase ketoreductases interact with acyl carrier protein-attached substrates. Moretto L, Vance S, Heames B & Broadhurst RW (2017)  Chemical Communications 53, 11457-11460. PMID: 28980673.

Sticky swinging arm dynamics: studies of an acyl carrier protein domain from the mycolactone polyketide synthase. Vance S, Tkachenko O, Thomas B, Bassuni M, Hong H, Nietlispach D & Broadhurst W (2016) Biochemical Journal 473, 1097-1110. PMID: 26920023.

The crystal structure of Clostridium perfringens SleM, a muramidase involved in cortical hydrolysis during spore germination. Al-Riyami B, Üstok FI, Stott K, Chirgadze DY, Christie G. (2016) Proteins 84(11):1681-1689. PMID: 27488615.

Crystal structure and molecular imaging of the Nav channel β3 subunit indicates a trimeric assembly. Namadurai S, Balasuriya D, Rajappa R, Wiemhöfer M, Stott K, Klingauf J, Edwardson JM, Chirgadze DY, Jackson AP (2014) J Biol Chem. 289(15):10797-811. PMID: 24567321.

Mutational analysis reveals a single binding interface between RhoA and its effector, PRK1. Hutchinson CL, Lowe PN, McLaughlin SH, Mott HR, Owen D. (2011), Biochemistry, 50, 2860–2869. PMID: 21351730.

Shared active site architecture between the large subunit of eukaryotic primase and DNA photolyase. Sauguet L, Klinge S, Perera RL, Maman JD, Pellegrini L. (2010), PLoS One, 5, e10083. PMID: 20404922.

Defining the molecular basis of BubR1 kinetochore interactions and APC/C-CDC20 inhibition. D’Arcy S, Davies OR, Blundell TL, Bolanos-Garcia VM. J Biol Chem (2010), 285, 14764-76. PMID: 20220147.

The sodium channel {beta}3-subunit induces multiphasic gating in NaV1.3 and affects fast inactivation via distinct intracellular regions. Cusdin FS, Nietlispach D, Maman J, Dale TJ, Powell AJ, Clare JJ, Jackson AP. (2010), J Biol Chem, 285, 33404-12. PMID: 20675377.

Structural comparison of two CSPG-binding DBL domains from the VAR2CSA protein important in malaria during pregnancy. Khunrae P, Philip JM, Bull DR, Higgins MK. (2009), J Mol Biol, 393, 202-13. PMID: 19695262.

An oligomeric signaling platform formed by the Toll-like receptor signal transducers MyD88 and IRAK-4. Motshwene PG, Moncrieffe MC, Grossmann JG, Kao C, Ayaluru M, Sandercock AM, Robinson CV, Latz E, Gay NJ. (2009), J Biol Chem, 284, 25404-11. PMID: 19592493.

The RNase E of Escherichia coli is a membrane-binding protein. Khemici V, Poljak L, Luisi BF, Carpousis AJ. (2008), Mol Microbiol, 70, 799-813. PMID: 18976283.

Assembly of oligomeric death domain complexes during Toll receptor signaling. Moncrieffe MC, Grossmann JG, Gay NJ. (2008), J Biol Chem, 283, 33447-54. PMID: 18829464.

Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome. Worrall JA, Górna M, Crump NT, Phillips LG, Tuck AC, Price AJ, Bavro VN, Luisi BF. (2008), J Mol Biol, 382, 870-83. PMID: 18691600.

Structural basis for inhibition of homologous recombination by the RecX protein. Ragone S, Maman JD, Furnham N, Pellegrini L. (2008), EMBO J, 27, 2259-69. PMID: 18650935.

Allosteric activation of the ATPase activity of the Escherichia coli RhlB RNA helicase. Worrall JA, Howe FS, McKay AR, Robinson CV, Luisi BF. (2008), J Biol Chem, 283, 5567-76. PMID: 18165229.

Autonomous folding of interdomain regions of a modular polyketide synthase. Richter CD, Stanmore DA, Miguel RN, Moncrieffe MC, Tran L, Brewerton S, Meersman F, Broadhurst RW, Weissman KJ. (2007), FEBS J, 274, 2196-209. PMID: 17419733.

Role of the Spatzle Pro-domain in the generation of an active toll receptor ligand. Weber AN, Gangloff M, Moncrieffe MC, Hyvert Y, Imler JL, Gay NJ. (2007), J Biol Chem, 282, 13522-31. PMID: 17324925.

Structural basis for the inhibition of activin signalling by follistatin. Harrington AE, Morris-Triggs SA, Ruotolo BT, Robinson CV, Ohnuma S, Hyvönen M. (2006), EMBO J, 25, 1035-45. PMID: 16482217.

Characterization of symmetric complexes of nerve growth factor and the ectodomain of the pan-neurotrophin receptor, p75NTR. Aurikko JP, Ruotolo BT, Grossmann JG, Moncrieffe MC, Stephens E, Leppänen VM, Robinson CV, Saarma M, Bradshaw RA, Blundell TL. (2005), J Biol Chem, 280, 33453-60. PMID: 16009712.

Solution structure of the isolated Pelle death domain. Moncrieffe MC, Stott KM, Gay NJ. (2005), FEBS Lett, 579, 3920-6. PMID: 16004997.

Engineering the structural stability and functional properties of the GI domain into the intrinsically unfolded GII domain of the yeast linker histone Hho1p. Sanderson A, Stott K, Stevens TJ, Thomas JO. (2005), J Mol Biol, 349, 608-20. PMID: 15878177.

Ligand-receptor and receptor-receptor interactions act in concert to activate signaling in the Drosophila toll pathway. Weber AN, Moncrieffe MC, Gangloff M, Imler JL, Gay NJ. (2005), J Biol Chem, 280, 22793-9. PMID: 15795223.

Distinct properties of the two putative “globular domains” of the yeast linker histone, Hho1p. Ali T, Thomas JO. (2004), J Mol Biol, 337, 1123-35. PMID: 15046982.

Quaternary structure and catalytic activity of the Escherichia coli ribonuclease E amino-terminal catalytic domain. Callaghan AJ, Grossmann JG, Redko YU, Ilag LL, Moncrieffe MC, Symmons MF, Robinson CV, McDowall KJ, Luisi BF. (2003), Biochemistry, 42, 13848-55. PMID: 14636052.

Crystal structures of the heparan sulfate-binding domain of follistatin. Insights into ligand binding. Innis CA, Hyvönen M. (2003), J Biol Chem, 278, 39969-77. PMID: 12867435.

Oct-1 POU and octamer DNA co-operate to recognise the Bob-1 transcription co-activator via induced folding. Chang JF, Phillips K, Lundbäck T, Gstaiger M, Ladbury JE, Luisi B. (1999), J Mol Biol, 288, 941-52. PMID: 10329190.

Two mutations in the HMG-box with very different structural consequences provide insights into the nature of binding to four-way junction DNA. Teo SH, Grasser KD, Hardman CH, Broadhurst RW, Laue ED, Thomas JO. (1995), EMBO J, 14, 3844-53. PMID: 7641702.